Manager Roy Hodgson will "re-configure" his 23-man squad now Barry has been ruled out by the stomach problem he suffered during Saturday's friendly win in Norway.
After scans confirmed Barry could not play any meaningful role in Poland and Ukraine this summer, Hodgson added Jagielka as an eighth defender in preference to Liverpool's (former Mackem) Jordan Henderson, who had been on standby in midfield.
Jagielka had appeared set to be part of Fabio Capello's World Cup squad in 2010 until he ruptured his cruciate ligament in April 2009.