John Alder and Liam Sweeney were on their way to watch their team in New Zealand last July when they died.
'United We Stand' was played at half time in today's fixture against Southampton, with the blessing of both families.
Last season, the club put a prompt on the big screens in the 17th minute to encourage supporters to applaud the two loyal fans.
Joyce Robbins, sister of John Alder- My family and I are very grateful to Newcastle United and the Club's supporters for all they have done in John and Liam's memory.
The applause on 17 minutes home and away has been one of many wonderful gestures, as is the song which will be played on Sunday.
Newcastle United was undoubtedly John's passion but he was also an extremely private man.
I'm sure he would be very happy to know that fans will be concentrating on supporting the team he loved so dearly and we wish everyone the best of luck for the new season.
Newcastle United have been magnificent and I'd like to say a big thank you to everyone at the Club.
It's an honour for the song to be played at the stadium where John and Liam both sat and watched the team.
It will be a proud moment to hear it played.