Jenas, 32, has been working a pundit for the BBC for the past two seasons and announced his retirement live on Radio 4's Today programme on Thursday morning.
'I have officially retired. I tried my best to come back from the knee injury but unfortunately that is the end of my career,' he said.
Jenas' last match came on March 15, 2014 in a 3-0 win for QPR over Yeovil at Loftus Road in a Championship fixture. He injured his anterior cruciate knee ligament in training the following month.
Jenas was released by QPR at the end of the 2013-14 season but was allowed to use the club's facilities in a bid to return to full fitness, one which ultimately failed.
JERMAINE JENAS CAREER STATS
Nottm Forest (2000-02): 33 games, 4 goals
Newcastle (2002-05): 152 games, 12 goals
Tottenham (2005-13): 202 games, 26 goals
Aston Villa (loan; 2011-12): 3 games
Nottm Forest (loan; 2012-13): 6 games, 1 goal
QPR (2013-15): 40 games, 4 goals
England (2003-09): 21 games 1 goal
Total: 457 games, 48 goals